HPLC Quantification of 4-Nitrophenol and its Conjugated Metabolites from Bile

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HPLC Quantification of 4-Nitrophenol and its Conjugated Metabolites from Bile

An isocratic ion pair RP-HPLC method with UV-Vis detection has been developed and validated for simultaneous analysis of 4-nitrophenol (PNP), 4-nitrophenyl β-glucuronide (PNP-G), and 4-nitrophenyl sulfate (PNP-S) in rat bile samples using 4-ethylphenol (ETP) as internal standard. Chromatographic separation was achieved on a C(18) column by isocratic elution with a mobile phase consisted of meth...

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Ginsenoside Rg1 (Rg1), the major effective component of ginseng, has been shown to have multiple bioactivities, but low oral bioavailability. The aim of this study was to develop a simple, sensitive and rapid high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, which could be used to validate and quantify the concentrations of Rg1 and its metabolites in Sprague-Daw...

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15 صفحه اول

Application of Excitation Sculpting in the Quantification of Conjugated Bile Acids in Bile

INTRODUCTION: Bile acids are major components of bile and are present mostly in conjugation with glycine and/or taurine. Amide bonds formed as a result of this conjugation provide well resolved ‘NH’ signals in the down-field region of the H MR spectrum of bile. However, at physiologic pH, these amide protons are in dynamic exchange with biliary water and show decreased signal intensity. Lowerin...

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BACKGROUND Therapeutic drug monitoring for hydroxychloroquine (HCQ) has been suggested to assess nonadherence and optimize treatment efficacy in systemic lupus erythematosus patients. MATERIALS & METHODS After protein precipitation, HCQ and its metabolites, desethylhydroxychloroquine and desethylchloroquine were separated on a phenyl column and monitored by fluorescence detection. The method ...

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ژورنال

عنوان ژورنال: Scientia Pharmaceutica

سال: 2011

ISSN: 0036-8709,2218-0532

DOI: 10.3797/scipharm.1106-22